General method for regulating protein stability with light.
نویسندگان
چکیده
Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
منابع مشابه
Oxidative stability, biochemical indices and characteristics of the fatty acid composition of fish protein isolated from tuna (Thunnus albacares) canning by-product stored at -24◦C for 6 months
Tuna processing by-product is estimated at more than 100,000 metric tonnes annually in Iran, which could be a potential source of edible protein in human food. Hence, recovering proteins from tuna by-products is a big achievement in the seafood industry. In this work tuna protein isolates (TPI) were extracted from dark/ red meat using the isoelectric solubilization/ precipitation method. Oxidat...
متن کاملSemi-Continuous Cultivation of Photosynthetic Cells in a Flat Plate Photobioreactor
From an engineering point of view, the effect of light intensity distribution on the stability of growth rate should be taken into account in designing effective photobioreactors and sustaining stable growth rates. In the experiments described here, in order to keep operational parameters at an almost constant level, a semi-continuous culture method was developed for cultivation of photosynthet...
متن کاملEffect of Time of Aerobic Exercise in the Light-dark Cycle on Glycemic Control, SIRT1 Protein Expression, and NAD+/NADH Ratio in Skeletal Muscle of Type 2 Diabetes Model Mice
Introduction: Mitochondrial function is regulated by the dark-light cycle under physiological and pathological conditions. Time-dependent exercise interventions may affect metabolic health in diabetic patients by regulating hyperglycemia. However, limited data are available about the correlation between the time of exercise and the regulation of muscle circadian rhythm in diabetes conditions. T...
متن کاملStudy of Optical Properties, Thermal Kinetic Decomposition and Stability of Coated PETN-Litholrubine nano-Composite via Solvent/None-Solvent Method Using Taguchi Experimental Design
In this research, in order to coating PETN particles, nano-pigment of redlitholrubine B 57:1 (NLR) was used in a surfactant environment of cetyltrimethylammonium bromide (CTAB) using solvent/none-solvent (water-acetone) method. Afterstructural studies of PETN-NLR nanocomposite by infrared (FT-IR) and field emissionscanning electron microscopy-Energy dispersive X-ray (FES...
متن کاملEffect of Microencapsulation on Photo-Stability of Nifedipine
Nifedipine (NIF), a 1,4-dihydropyridine calcium channel antagonist, undergoes photo-degradation to dehydro-nifedipine (DNIF) upon exposure to ultraviolet (UV) light and to the nitroso analogue of dehydro-nifedipine (NDNIF) when exposed to sunlight or some kinds of artificial lights. NIF photo-degradation products do not contribute to clinical activity, thus prevention of photo-degradation of NI...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- ACS chemical biology
دوره 9 1 شماره
صفحات -
تاریخ انتشار 2014